CollabrativeTSRC.ppt

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  A Collaborative Investigation of Two Tobacco TSNA Methods June B. Reece, Charles H. Risner and Walter T. Morgan  Tobacco Specific Nitrosamine Collaborative Study  Background Method 1 -QUANTIFICATION OF TOBACCO SPECIFIC NITROSAMINES IN TOBACCO USING ALKALINE METHYLENE CHLORIDE EXTRACTIONMethod 2 -QUANTIFICATION OF TOBACCO SPECIFIC NITROSAMINES IN TOBACCO USING BUFFER EXTRACTION  Tobacco Specific Nitrosamine Collaborative Study  Background ã Method 1 -Quantification of Tobacco Specific Nitrosamines in Tobacco Using Alkaline Methylene Chloride Extraction ã Best Day-to-Day Reproducibility (Robotic Preparation) ã Lowest Within-day Standard Deviation (Robotic Preparation) ã Minimal Solvent ã Economical Extraction ã High Sample Throughput ã Three Laboratories Performing Similar Methodologies  Tobacco Specific Nitrosamine Collaborative Study  Background ã Method 2 -Quantification of Tobacco Specific Nitrosamines in Tobacco Using Buffer Extraction ã Basic Method Utilized by 6 Laboratories in the 2000 Study
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